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The purpose of this research was to prepare functional cell-targeting magnetic nanoparticles (MCMNPs) containing superparamagnetic iron oxide nanoparticles (SPIONs). Nanoparticles synthesized from SPIONs and hydrophilic di-galactose-dextran (DC-10L) were coated with the hydrophilic polysaccharide chitosan to confer function. This was verified by the measurements of zeta potential and the analysis of FTIR spectroscopy. The ability of the MCMNPs to interact with THP-1, the monocytic cell line, was determined by the measurement of zeta potential. The cell-targeting effect of the MCMNPs, by themselves, was confirmed by the measurement of cell uptake, viability, and cell apoptosis. The interaction between nanoparticles and cells was time dependent and the internalization rate of MCMNPs into THP-1 cells was higher than that of non-targeted MNPs, because of the specific recognition between the chitosan and the membrane-bound galactose-specific lectin. In contrast, the cell viability of the MCMNPs was equal to that of MNPs as the presence of chitosan could protect the nanoparticles from the ROS generated by the cells.Country Music News International Magazine is the place where you find country music news, interviews, cd reviews and many more in German and English. Keep up to date with your favorite country star by reading Country Music News International Magazine.
Discover the cheats and tips on how to have the most farm animals in Farming Simulator 14. Plus, an in-depth overview of all the new features.
Farm Simulator 14 Season Pass Release
The Farming Simulator 14 Season pass is available on the market for pre-order. It’s a €99.99 pre-order bonus including all in game content that will be released for the game in the year 2015.
The Farming Simulator 14 Season pass will have all content available in the game over the course of the season, that includes:
New cars and new trailers: New cars have been added this year to the Farming Simulator series. A large variety of tractors, harvesters and trailers, from industry leading manufacturers such as Claas, Case